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This study was conducted to determine the effects of the artificial culture medium of wild-ginsengs on in vitro fermentation characteristics. NH_(３)-N concentration was showed the highest in 3％ WGM treatment among all treatments and control. In addition, microbila protein synthesis was significantly different in all treatments throughout the incubation time, and WGM 3％ tratment was the highest at the 9 h incubation(P＜0.05). Protozoa numbers within rumen were decreased in all WGM treatments a t9h incubation time, whereas WGM 3％ treatment was always decreased throughtout the incubation(P＜0.05). NDF and ADF digestibility showed no significantly increased as the incubation time in both control and treatments. NDF digestibility showed no significantly increased as the incubation time in both control and treatments. NDF digestibility showed no significantly difference between contro and the 3％ treatment, and ADF digestibility was similar in all. Total volatile fatty acid(VFA) concentrations of WGM treatments without 5％ were significantly higher than control(P＜0.05). No differences were observed in total VFA, acetate, propionate and butyrate concentration among the WGM treatments. Acetate／Propionate roatio of WGN treatments was higher than control after 12 h incubation(P＜0.05). As a result of the artificial culture medium of wild-ginseng on rumen fermentation characteristics in vitro, microbial protein synthesis of WGM treatment was higher than control, and WGM 3％ was the highest in all treatments(P＜0.05). The effect of saponin in artificaial culture medium of wild-ginseng tended to decrease NH_(３)-N concentration, while it increases the microbial synthesis in early incubation. Therefor, artificial cultures medium of wild-ginseng ca increase utilization of feed by microbial and anti-protozoal effects of saponin, which may enhance microbial synthesis capacity in early fernentation period in rumen.
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